Calsequestrin 2 Antibody (PSH03-16)

Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102]

Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102]

Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunofluorescence analysis of paraffin-embedded human skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32102, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102]

Western Blot: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Western blot analysis of Calsequestrin 2 on different lysates with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/10,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate (15 µg/Lane)
Lane 2: Mouse heart tissue lysate (15 µg/Lane)
Lane 3: Mouse brain tissue lysate (negative) (15 µg/Lane)
Lane 4: Rat skeletal muscle tissue lysate (15 µg/Lane)
Lane 5: Rat heart tissue lysate (15 µg/Lane)
Lane 6: Rat brain tissue lysate (negative) (15 µg/Lane)

Predicted band size: 46 kDa
Observed band size: 55 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.