CaM Kinase I Antibody (4P4K9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16210

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16210-100ul
NBP3-16210-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4P4K9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase I Antibodies »

Product Summary for CaM Kinase I Antibody (4P4K9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CaM Kinase I (NP_003647.1). MLGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKEALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase I Antibody (4P4K9)

Western Blot: CaM Kinase I Antibody (4P4K9) [NBP3-16210]

Western Blot: CaM Kinase I Antibody (4P4K9) [NBP3-16210]

Western Blot: CaM Kinase I Antibody (4P4K9) [NBP3-16210] - Western blot analysis of extracts of various cell lines, using CaM Kinase I Rabbit mAb (NBP3-16210) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Applications for CaM Kinase I Antibody (4P4K9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase I

Ca(+2)/calmodulin-dependent protein kinases (CaMKs) are activated upon binding of Ca(+2)/calmodulin. To gain maximal activity, CaMK I and CaMK IV can be further phosphorylated by an upstream kinase, CaMK kinase (CaMKK) (1). CaMKI is a Ca2+/calmodulin-dependent protein kinase that is widely expressed in eukaryotic cells and tissues but for which few, if any, physiological substrates are known (2). CaMKI has been shown to comprise a family of four structurally related isoforms (alpha, beta, gamma, and delta) encoded by separate genes with abundant expression in mature brain. In mouse brain, the four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. CaMKI may also have possible functional involvement of in the basal dendritic growth of hippocampal neurons (3)

Long Name

Calcium/Calmodulin-dependent Protein Kinase I

Alternate Names

calcium/calmodulin-dependent protein kinase I, calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM kinase I alpha, CAMKI, caM-KI, caMKI-alpha, EC 2.7.11, EC 2.7.11.17, MGC120317, MGC120318

Gene Symbol

CAMK1

Additional CaM Kinase I Products

Product Documents for CaM Kinase I Antibody (4P4K9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase I Antibody (4P4K9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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