CaM Kinase II alpha [p Thr286] Antibody (SR1853)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-22608

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-22608-100ul
NBP3-22608-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1853

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase II alpha Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-CaM Kinase II alpha [Thr286] (Uniprot #: Q9UQM7, P11798, P11275)

Modification

p Thr 286

Specificity

Detects endogenous levels of total phospho-CaM Kinase II alpha [Thr286]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase II alpha [p Thr286] Antibody (SR1853)

Western Blot: CaM Kinase II alpha [p Thr 286] Antibody (SR1853) [NBP3-22608] -

Western Blot: CaM Kinase II alpha [p Thr 286] Antibody (SR1853) [NBP3-22608] - Western blot analysis using NBP3-22608 in Mouse brain treated with Lambda phosphatase cell lysate.

Applications for CaM Kinase II alpha [p Thr286] Antibody (SR1853)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Gene Symbol

CAMK2A

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha [p Thr286] Antibody (SR1853)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase II alpha [p Thr286] Antibody (SR1853)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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