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CaM Kinase II alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81830

Novus Biologicals, part of Bio-Techne
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NB100-81830
NB100-81830-0.025ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human CaM Kinase II alpha around amino acids 284~288 (Q-E-T-V-D).

Specificity

Detects endogenous levels of total CaMKII protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CaM Kinase II alpha Antibody

Western Blot: CaM Kinase II alpha Antibody [NB100-81830] -

Western Blot: CaM Kinase II alpha Antibody [NB100-81830] - Analysis of extracts from Hela using NB100-81830

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NB100-81830] -

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NB100-81830] - Staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using NB100-81830
CaM Kinase II alpha Antibody

Western Blot: CaM Kinase II alpha Antibody [NB100-81830] -

Western Blot: CaM Kinase II alpha Antibody [NB100-81830] - Western blotting analysis using NB100-81830

Applications for CaM Kinase II alpha Antibody

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28912554).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Entrez Gene IDs

815 (Human); 12322 (Mouse); 25400 (Rat)

Gene Symbol

CAMK2A

UniProt

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CaM Kinase II alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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