CaM Kinase II alpha [p Thr286] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-184

Novus Biologicals, part of Bio-Techne
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NB300-184
NB300-184-0.02ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit

Cited:

Human

Predicted:

Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase II alpha Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH. Accession # P11275

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28972541).

Modification

p Thr286

Localization

Cell membrane; presynaptic membrane. Cell junction; synapse.

Specificity

Specific for endogenous levels of the ~50 kDa CaM Kinase II alpha and the ~60 kDa beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CaM Kinase II alpha [p Thr286] Antibody

Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NB300-184]

Western Blot: CaM Kinase II alpha [p Thr286] Antibody [NB300-184]

Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr286 CaM Kinase II. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for CaM Kinase II alpha [p Thr286] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28972541).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.9 using NB300-184 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaM KII further autophosphorylates itself at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the other hand, PKA (protein kinase A) prevents this phosphorylation process through its inhibitory effect on PP1.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Entrez Gene IDs

815 (Human); 12322 (Mouse); 25400 (Rat)

Gene Symbol

CAMK2A

UniProt

P11275 (Rat)

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha [p Thr286] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase II alpha [p Thr286] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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