CaM Kinase II alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46796

Novus Biologicals, part of Bio-Techne
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NBP2-46796
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CaM Kinase II alpha Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LYQQIKAGAYDFPSPEWDTVTPEAKDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaM Kinase II alpha Antibody

Western Blot: CaM Kinase II alpha Antibody [NBP2-46796]

Western Blot: CaM Kinase II alpha Antibody [NBP2-46796]

Western Blot: CaM Kinase II alpha Antibody [NBP2-46796] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody [NBP2-46796]

Immunocytochemistry/ Immunofluorescence: CaM Kinase II alpha Antibody [NBP2-46796]

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP2-46796] - Staining of human cell line U-2 OS shows localization to plasma membrane.
Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP2-46796]

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP2-46796]

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP2-46796] - Staining of human hippocampus shows moderate positivity in subsets of neuronal cells.

Applications for CaM Kinase II alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaM Kinase II alpha

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Long Name

Calcium/Calmodulin-dependent Protein Kinase II alpha

Alternate Names

CAMK2A

Entrez Gene IDs

817 (Human)

Gene Symbol

CAMK2D

UniProt

Q13557

Additional CaM Kinase II alpha Products

Product Documents for CaM Kinase II alpha Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaM Kinase II alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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