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CaM Kinase II [p Thr306] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60767

Novus Biologicals, part of Bio-Techne
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NBP2-60767

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CaM Kinase II [p Thr306] Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding CaM Kinase II conjugated to KLH. Accession # P11310

Modification

p Thr306

Specificity

Specific for endogenous levels of the ~50 kDa alpha CaM Kinase II and the ~60 kDa beta CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with lambda-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CaM Kinase II [p Thr306] Antibody

Western Blot: CaM Kinase II [p Thr306] Antibody [NBP2-60767]

Western Blot: CaM Kinase II [p Thr306] Antibody [NBP2-60767]

Western Blot: CaM Kinase II [p Thr306] Antibody [NBP2-60767] - specific immunolabelingof the ~50 kDa alpha and the ~60 kDa Beta CaM Kinase IIphosphorylated at Thr306 in the first lane (-). Phosphospecificity isshown in the second lane (+) where the immunolabeling iscompletely eliminated by lysate treatment with lambdaphosphatase (Lamba-Ptase, 800 units/1mg protein for 30 minutes).

Applications for CaM Kinase II [p Thr306] Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Application Notes
WB analysis shows bands at 50/60 kDa
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CaM Kinase II

Calmodulin kinase II (CaMKII) is an 8 - 12 subunit multimeric calcium/calmodulin dependent Ser/Thr protein kinase. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes (alpha, beta, gamma, delta), each of which contains a catalytic, autoregulatory, and subunit-association domain.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II

Alternate Names

CAMK2, CAMK2B

Gene Symbol

CAMK2B

Additional CaM Kinase II Products

Product Documents for CaM Kinase II [p Thr306] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CaM Kinase II [p Thr306] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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