Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoblotting, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 22B1

Concentration

1 mg/ml

View all CaMKII alpha/beta Antibodies »

Product Specifications

Immunogen

Synthetic peptide

Modification

p Thr286, p Thr287

Localization

Cytoplasm , Mitochondrion , Nucleus , Cell Junction , Synapse , Presynaptic Cell Membrane

Specificity

This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)

Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII alpha/beta protein using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000. Analysis of CaMKII alpha/beta and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.
Immunocytochemistry/Immunofluorescence: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [NB110-96869] - Immunohistochemistry analysis using Mouse Anti-CaMKII alpha/beta Monoclonal Antibody, Clone 22B1 (NB110-96869). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII alpha/beta Monoclonal Antibody (NB110-96869) at 1:5000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.
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Applications for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
1 ug/ml was sufficient for detection of 0.2 ug CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.Use in immunoblotting reported in scientific literature (PMID: 27599291).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaMKII alpha/beta

CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho- CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Auto-phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

Alternate Names

calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, calcium/calmodulin-dependent protein kinase II alpha, calcium/calmodulin-dependent protein kinase II alpha-B subunit, CaM kinase II alpha subunit, CaM kinase II subunit alpha, CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha, CaMK-II alpha subunit, CaMK-II subunit alpha, CaMKIINalpha, CaM-kinase II alpha chain, EC 2.7.11, EC 2.7.11.17, KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Entrez Gene IDs

815 (Human); 25400 (Rat)

Gene Symbol

CAMK2A

UniProt

P11275 (Rat)

Additional CaMKII alpha/beta Products

Product Documents for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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