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CaMKII beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88212

Novus Biologicals, part of Bio-Techne
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NBP1-88212
NBP1-88212-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CaMKII beta Antibody

Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP1-88212]

Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP1-88212]

Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP1-88212] - Analysis in human cerebral cortex and placenta tissues. Corresponding RNA-seq data are presented for the same tissues
Western Blot: CaMKII beta Antibody [NBP1-88212]

Western Blot: CaMKII beta Antibody [NBP1-88212]

Western Blot: CaMKII beta Antibody [NBP1-88212] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry: CaMKII beta Antibody [NBP1-88212]

Immunohistochemistry: CaMKII beta Antibody [NBP1-88212]

Immunohistochemistry: CaMKII beta Antibody [NBP1-88212] - Staining of mouse brain shows moderate to strong positivity in more than 75 % of neurons in the cerebral cortex.

Applications for CaMKII beta Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:5

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaMKII beta

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene.

Long Name

Calcium/calmodulin-dependent protein kinase type II subunit beta

Alternate Names

CAM2, CAMKB, EC 2.7.11.17

Gene Symbol

CAMK2B

Additional CaMKII beta Products

Product Documents for CaMKII beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CaMKII beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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