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CaMKII beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13157

Novus Biologicals, part of Bio-Techne
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NBP3-13157

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CaMKII beta. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CaMKII beta Antibody

Western Blot: CaMKII beta Antibody [NBP3-13157]

Western Blot: CaMKII beta Antibody [NBP3-13157]

Western Blot: CaMKII beta Antibody [NBP3-13157] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII beta antibody (NBP3-13157) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP3-13157]

Immunocytochemistry/ Immunofluorescence: CaMKII beta Antibody [NBP3-13157]

Immunocytochemistry/Immunofluorescence: CaMKII beta Antibody [NBP3-13157] - CaMKII beta antibody detects CaMKII beta protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CaMKII beta protein stained by CaMKII beta antibody (NBP3-13157) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP3-13157]

Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP3-13157]

Immunohistochemistry-Paraffin: CaMKII beta Antibody [NBP3-13157] - CaMKII beta antibody detects CAMK2B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CaMKII beta antibody (NBP3-13157) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for CaMKII beta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CaMKII beta

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene.

Long Name

Calcium/calmodulin-dependent protein kinase type II subunit beta

Alternate Names

CAM2, CAMKB, EC 2.7.11.17

Gene Symbol

CAMK2B

Additional CaMKII beta Products

Product Documents for CaMKII beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CaMKII beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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