CaMKIV Antibody (1G4P4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16802
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1G4P4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CaMKIV Antibody (1G4P4)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 374-473 of human CAMKIV (Q16566). EGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CaMKIV Antibody (1G4P4)
Western Blot: CaMKIV Antibody (1G4P4) [NBP3-16802]
Western Blot: CaMKIV Antibody (1G4P4) [NBP3-16802] - Western blot analysis of extracts of various cell lines, using CaMKIV antibody (NBP3-16802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunocytochemistry/ Immunofluorescence: CaMKIV Antibody (1G4P4) [NBP3-16802]
Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunofluorescence analysis of U-2 OS cells using CaMKIV Rabbit mAb (NBP3-16802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: CaMKIV Antibody (1G4P4) [NBP3-16802]
Immunohistochemistry-Paraffin: CaMKIV Antibody (1G4P4) [NBP3-16802] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaMKIV Rabbit mAb (NBP3-16802) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for CaMKIV Antibody (1G4P4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CaMKIV
Alternate Names
brain Ca(2+)-calmodulin-dependent protein kinase type IV, brain Ca++-calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase IV, calcium/calmodulin-dependent protein kinase type IV, calcium/calmodulin-dependent protein kinase type IV catalytic chain, CAM kinase- GR, CAM kinase IV, CaM kinase-GR, caMK, CaMK IV, CaMK-GR, EC 2.7.11, EC 2.7.11.17, IV, MGC36771
Gene Symbol
CAMK4
Additional CaMKIV Products
Product Documents for CaMKIV Antibody (1G4P4)
Product Specific Notices for CaMKIV Antibody (1G4P4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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