CaMKIV Antibody

Western Blot: CaMKIV Antibody [NBP1-78002]

Western Blot: CaMKIV Antibody [NBP1-78002] - Shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

Immunohistochemistry-Paraffin: CaMKIV Antibody [NBP1-78002]

Immunohistochemistry-Paraffin: CaMKIV Antibody [NBP1-78002] - Antibody was diluted 1:500 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

CaMKIV Antibody

Immunohistochemistry of Anti-CAMK4 antibody. Tissue: human brain cortex was formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-CaM Kinase IV was diluted 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.