CaMKK alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42683
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 455 and 505 of human calcium/calmodulin-dependent protein kinase kinase 1, alpha using the numbering given in entry NP_757343.1 (GeneID 84254).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CaMKK alpha Antibody
![Western Blot: CaMKK alpha Antibody [NBP1-42683]](https://resources.bio-techne.com/images/products/CaMKK-alpha-Antibody-Western-Blot-NBP1-42683-img0003.jpg "Western Blot: CaMKK alpha Antibody [NBP1-42683]")
Western Blot: CaMKK alpha Antibody [NBP1-42683]
Western Blot: CaMKK alpha Antibody [NBP1-42683] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CaMKK alpha antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CaMKK alpha was also immunoprecipitated by rabbit anti-CaMKK alpha antibody NBP1-42682, which recognizes an upstream epitope.![Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683]](https://resources.bio-techne.com/images/products/CaMKK-alpha-Antibody-Immunohistochemistry-NBP1-42683-img0005.jpg "Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683]")
Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683]
Immunohistochemistry-Paraffin: CaMKK alpha Antibody [NBP1-42683] - Section of human lung carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti- CaMKK alpha used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for CaMKK alpha Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100- 1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CaMKK alpha
Long Name
Calcium/Calmodulin-dependent Protein Kinase Kinase alpha
Alternate Names
CaM-kinase IV kinase, CaMKK1
Entrez Gene IDs
84254 (Human)
Gene Symbol
CAMKK1
Additional CaMKK alpha Products
Product Documents for CaMKK alpha Antibody
Product Specific Notices for CaMKK alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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