Canine CCL5/RANTES Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB60451

R&D Systems, part of Bio-Techne
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MAB60451
MAB60451-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Canine

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 676106

View all CCL5/RANTES Antibodies »

Product Summary for Canine CCL5/RANTES Antibody

Immunogen

E. coli-derived recombinant canine CCL5/RANTES
Ser24-Ser91
Accession # Q8HYS0

Specificity

Detects canine CCL5/RANTES in direct ELISAs. In Western blots, no cross-reactivity with recombinant CCL5/RANTES from cat, cotton rat, mouse, or human is observed. No cross-reactivity with recombinant mouse CCL7/MARC, rmCCL17/TARC, recombinant human (rh) CCL18/PARC, or rhCCL17/TARC is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Canine CCL5/RANTES Antibody

CCL5/RANTES antibody in Canine PBMCs by Immunocytochemistry (ICC).

CCL5/RANTES in Canine PBMCs.

CCL5/RANTES was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Canine CCL5/RANTES Monoclonal Antibody (Catalog # MAB60451) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Canine CCL5/RANTES Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta-chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1‑3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature canine CCL5 shares 74%‑84% amino acid seqeuence identity with cotton rat, feline, human, mouse, and rat CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (5, 6). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (7). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (8). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (9). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (10‑12). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (13‑15). CCL5 is up‑regulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (16).

References

  1. Schall, T.J. et al. (1990) Nature 347:669.
  2. Bacon, K.B. et al. (1995) Science 269:1727.
  3. Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
  4. Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
  5. Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
  6. Levy, J.A. (2009) J. Immunol. 182:3945.
  7. Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
  8. DeVico, A.L. and Gallo, R.C. (2004) Nat. Rev. Microbiol. 2:401.
  9. Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
  10. Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
  11. Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. 100:1885.
  12. von Hundelshausen, P. et al. (2005) Blood 105:924.
  13. von Hundelshausen, P. et al. (2001) Circulation 103:1772.
  14. Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
  15. Baltus, T. et al. (2003) Blood 102:1985.
  16. Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

Alternate Names

RANTES, SISd

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Gene Symbol

CCL5

UniProt

Q8HYS0

Additional CCL5/RANTES Products

Product Documents for Canine CCL5/RANTES Antibody

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Product Specific Notices for Canine CCL5/RANTES Antibody

For research use only

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