Canine CD4 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2410P

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Canine

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 296712

View all CD4 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant canine CD4
Val25-Lys401
Accession # P33705

Specificity

Detects canine CD4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD4, recombinant mouse CD4, and recombinant feline CD4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Canine CD4 PE-conjugated Antibody

Detection of CD4 antibody in Lymphocytes antibody in Canine PBMCs antibody by Flow Cytometry.

Detection of CD4 in Lymphocytes in Canine PBMCs by Flow Cytometry.

Lymphocytes in canine peripheral blood mononuclear cells were stained with Mouse Anti-Canine CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB2410P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Canine CD4 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Lymphocytes in canine peripheral blood mononuclear cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using FAB2410P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD4

CD4 is a 56-58 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed on a variety of cell types including T helper cells, follicular helper cells, neurons, T regulatory cells, microglia, and monocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. Over aa 25-401, canine CD4 shares 56% amino acid identity with human CD4.

Alternate Names

CD4

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Gene Symbol

CD4

UniProt

P33705

Additional CD4 Products

Product Documents for Canine CD4 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Canine CD4 PE-conjugated Antibody

For research use only

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