Canine/Feline SCF/c-kit Ligand Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2268
Key Product Details
Species Reactivity
Canine, Feline
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 371907
Product Specifications
Immunogen
E. coli-derived recombinant canine SCF/c‑kit Ligand
Lys26-Ala190
Accession # Q06220
Lys26-Ala190
Accession # Q06220
Specificity
Detects canine and feline SCF/c‑kit Ligand in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50%‑100% cross‑reactivity with recombinant human (rh) SCF and recombinant mouse SCF is observed and no cross-reactivity with rhM-CSF is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Canine/Feline SCF/c-kit Ligand Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Canine SCF/c-kit Ligand (Catalog # 2278-SC)
Recombinant Feline SCF/c-kit Ligand (Catalog # 2268-SC)
Sample: Recombinant Canine SCF/c-kit Ligand (Catalog # 2278-SC)
Recombinant Feline SCF/c-kit Ligand (Catalog # 2268-SC)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCF/c-kit Ligand
SCF, also known as c-kit ligand, is a 28 kDa‑40 kDa type I transmembrane (TM) glycoprotein that plays important roles in fetal and adult developmental processes. Endothelial cells, fibroblasts, and keratinocytes can express SCF. The 190 aa extracellular region contains a proteolytic cleavage site, generating 165 aa circulating monomers and noncovalent homodimers. Soluble feline and canine SCF share 93% aa identity with each other and 88%‑92% aa identity with pig, cow, sheep, goat, horse, and mink SCF.
Long Name
Stem Cell Factor
Alternate Names
c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF
Gene Symbol
KITLG
UniProt
Additional SCF/c-kit Ligand Products
Product Documents for Canine/Feline SCF/c-kit Ligand Antibody
Product Specific Notices for Canine/Feline SCF/c-kit Ligand Antibody
For research use only
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