CapG Antibody (1F1)

Novus Biologicals, part of Bio-Techne | Catalog # H00000822-M01

Novus Biologicals, part of Bio-Techne
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H00000822-M01-100ug
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CapG Antibodies »

Product Specifications

Immunogen

CAPG (AAH00728.1, 1 a.a. ~ 348 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSHLHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGGVESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGGKSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGFISRMQYAPNTQVEILPQGRESPIFKQFFKDWK

Specificity

CAPG - capping protein (actin filament), gelsolin-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CapG Antibody (1F1)

Western Blot: CapG Antibody (1F1) [H00000822-M01]

Western Blot: CapG Antibody (1F1) [H00000822-M01]

Western Blot: CapG Antibody (1F1) [H00000822-M01] - CAPG monoclonal antibody (M01), clone 1F1 Analysis of CAPG expression in HL-60.
Immunocytochemistry/ Immunofluorescence: CapG Antibody (1F1) [H00000822-M01]

Immunocytochemistry/ Immunofluorescence: CapG Antibody (1F1) [H00000822-M01]

Immunocytochemistry/Immunofluorescence: CapG Antibody (1F1) [H00000822-M01] - Analysis of monoclonal antibody to CAPG on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CapG Antibody (1F1) [H00000822-M01] - Detection limit for recombinant GST tagged CAPG is approximately 0.1ng/ml as a capture antibody.

Applications for CapG Antibody (1F1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CapG

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described.

Long Name

Capping Protein, Gelsolin-like

Alternate Names

AFCP

Entrez Gene IDs

822 (Human)

Gene Symbol

CAPG

OMIM

153615 (Human)

UniProt

AAH00728 (Human)

Additional CapG Products

Product Documents for CapG Antibody (1F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CapG Antibody (1F1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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