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CapG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15259

Novus Biologicals, part of Bio-Techne
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NBP2-15259

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (91%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CapG Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human CAPG. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus; Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CapG Antibody

Immunohistochemistry-Paraffin: CapG Antibody [NBP2-15259]

Immunohistochemistry-Paraffin: CapG Antibody [NBP2-15259]

Immunohistochemistry-Paraffin: Actin Regulatory Protein Antibody [NBP2-15259] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.
Western Blot: CapG Antibody [NBP2-15259]

Western Blot: CapG Antibody [NBP2-15259]

Western Blot: Actin Regulatory Protein Antibody [NBP2-15259] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE, antibody diluted at 1:2000.
CapG Antibody

Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP2-15259] -

Immunocytochemistry/ Immunofluorescence: CapG Antibody [NBP2-15259] - CapG antibody [N3C3] detects CapG protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CapG protein stained by CapG antibody [N3C3] (NBP2-15259) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for CapG Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CapG

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed, but have not been fully described. [provided by RefSeq]

Long Name

Capping Protein, Gelsolin-like

Alternate Names

AFCP

Gene Symbol

CAPG

UniProt

Additional CapG Products

Product Documents for CapG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CapG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.