Capicua Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-59905

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Capicua Antibodies »

Product Specifications

Immunogen

The epitope recognized by this antibody maps to a region within residue 1150-1200 of human Capicua. [NP_055940.3]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

164 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Capicua Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Capicua Antibody - BSA Free [NB110-59905]

Immunocytochemistry/ Immunofluorescence: Capicua Antibody - BSA Free [NB110-59905]

Immunocytochemistry/Immunofluorescence: Capicua Antibody [NB110-59905] - Capicua antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunoprecipitation: Capicua Antibody - BSA Free [NB110-59905]

Immunoprecipitation: Capicua Antibody - BSA Free [NB110-59905]

Immunoprecipitation: Capicua Antibody [NB110-59905] - Detection of Human CIC/Capicua by WB of IPs. Whole cell lysate from HeLa cells. Affinity purified rabbit NB110-59905 used for IP at 3 mcg/mg lysate. CIC was also IPed by rabbit anti-CICa antibody.

Applications for Capicua Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
This Capicua antibody is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. It is not recommended for Western blot analysis. With ICC/IF nuclear staining was observed in Neuro2A cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Capicua

Capicua is a transcriptional repressor protein belonging to HMG-box proteins which has been suggested to play a key role in CNS development and is expressed in foetal brain as well as medulloblastomas. Capicua protein expression levels are subject to posttranscriptional regulation by signaling downstream of two RTKs - EGFR and Torso (that propagate their signals through Ras-Raf-MAPK signaling cascade) and MAPK-mediated phosphorylation promotes capicua degradation. Capicua has been shown to act as a transcriptional repressor through interaction with a co-repressor Groucho. Moreover, Bantam which is a Hippo pathway player has recently been shown to acts in a feedback loop to limit Capicua expression for regulating EGFR signaling. Capicua is well conserved in mammals, and mediates RTK-dependent responses that are linked to cell proliferation, cell differentiation and cancer. Capicua can interact with ATXN1 as well as ATXN1L, and has been shown to be involved in Spinocerebellar Ataxia Type 1 (SCA-1).

Alternate Names

capicua homolog (Drosophila), KIAA0306capicua (Drosophila) homolog, protein capicua homolog

Entrez Gene IDs

23152 (Human); 71722 (Mouse); 308435 (Rat)

Gene Symbol

CIC

UniProt

Q96RK0 (Human)

Additional Capicua Products

Product Documents for Capicua Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Capicua Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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