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CAPZB Antibody (4H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00000832-M03

Novus Biologicals, part of Bio-Techne
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H00000832-M03

Key Product Details

Species Reactivity

Validated:

Human, Bovine

Cited:

Bovine

Applications

Validated:

Dot Blot, ELISA, Western Blot

Cited:

Cytometric Bead Assay Standard, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CAPZB (NP_004921, 192 a.a. ~ 272 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLTRQMEKDETVSDCSPHIANIGRLVEDMENKIRSTLNEIYFGKTKDIVNGLRSVQTFADKSKQEALKNDLVEALKRKQQC

Reactivity Notes

Bovine reported in scientific literature (PMID: 26344128).

Specificity

CAPZB - capping protein (actin filament) muscle Z-line, beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CAPZB Antibody (4H8)

Western Blot: CAPZB Antibody (4H8) [H00000832-M03]

Western Blot: CAPZB Antibody (4H8) [H00000832-M03]

Western Blot: CAPZB Antibody (4H8) [H00000832-M03] - Analysis of CAPZB expression in HeLa (Cat # L013V1).
Western Blot: CAPZB Antibody (4H8) [H00000832-M03]

Western Blot: CAPZB Antibody (4H8) [H00000832-M03]

Western Blot: CAPZB Antibody (4H8) [H00000832-M03] - analysis of CAPZB expression in transfected 293T cell line by CAPZB monoclonal antibody (M03), clone 4H8. Lane 1: CAPZB transfected lysate (9 KDa). Lane 2: Non-transfected lysate.
ELISA: CAPZB Antibody (4H8) [H00000832-M03]

ELISA: CAPZB Antibody (4H8) [H00000832-M03]

ELISA: CAPZB Antibody (4H8) [H00000832-M03] - Detection limit for recombinant GST tagged CAPZB is approximately 0.1ng/ml as a capture antibody.

Applications for CAPZB Antibody (4H8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for ELISA. Use in Dot blot reported in scientific literature (PMID: 26344128).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CAPZB

CAPZB is a member of the F-actin capping protein family. This gene encodes the beta subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.

Alternate Names

Cap Z, CAPB, CAPPB, capping protein (actin filament) muscle Z-line, beta, CAPZ, CapZ beta, F-actin capping protein beta subunit, F-actin-capping protein subunit beta, MGC104401, MGC129749, MGC129750

Entrez Gene IDs

832 (Human)

Gene Symbol

CAPZB

OMIM

601572 (Human)

Additional CAPZB Products

Product Documents for CAPZB Antibody (4H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CAPZB Antibody (4H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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