Carbohydrate Sulfotransferase 15/CHST15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88367

Novus Biologicals, part of Bio-Techne
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NBP1-88367
NBP1-88367-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carbohydrate Sulfotransferase 15/CHST15 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQASSAKEQSKMNTIIIGEASASTMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPNARLIVMLRDPVER

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23457544)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbohydrate Sulfotransferase 15/CHST15 Antibody

Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367]

Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367]

Immunocytochemistry/Immunofluorescence: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367] - Staining of human cell line A-431 shows localization to cytosol & centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367]

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367]

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP1-88367] - Staining of human heart muscle shows strong positivity in myocytes.

Applications for Carbohydrate Sulfotransferase 15/CHST15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbohydrate Sulfotransferase 15/CHST15

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxylgroup of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containingGlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence,GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found inthrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated earlyactivation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAGexpression; however such results are unclear in vivo

Alternate Names

BRAG, CHST15, GalNAc4S-6ST

Gene Symbol

CHST15

Additional Carbohydrate Sulfotransferase 15/CHST15 Products

Product Documents for Carbohydrate Sulfotransferase 15/CHST15 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbohydrate Sulfotransferase 15/CHST15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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