Carbohydrate Sulfotransferase 15/CHST15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15896

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carbohydrate Sulfotransferase 15/CHST15 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Carbohydrate Sulfotransferase 15/CHST15. The exact sequence is proprietary.

Localization

Golgi apparatus membrane; Single-pass type II membrane protein (Probable)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carbohydrate Sulfotransferase 15/CHST15 Antibody

Carbohydrate Sulfotransferase 15/CHST15 Antibody

Western Blot: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP2-15896] -

Western Blot: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP2-15896] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbohydrate Sulfotransferase 15/CHST15 antibody [N3C2], Internal (NBP2-15896) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP2-15896]

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP2-15896]

Immunohistochemistry-Paraffin: Carbohydrate Sulfotransferase 15/CHST15 Antibody [NBP2-15896] - Human gastric cancer, using CHST15 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for Carbohydrate Sulfotransferase 15/CHST15 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carbohydrate Sulfotransferase 15/CHST15

Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Alternate Names

BRAG, CHST15, GalNAc4S-6ST

Gene Symbol

CHST15

UniProt

Q7LFX5

Additional Carbohydrate Sulfotransferase 15/CHST15 Products

Product Documents for Carbohydrate Sulfotransferase 15/CHST15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carbohydrate Sulfotransferase 15/CHST15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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