Carbohydrate Sulfotransferase 2/CHST2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55604

Novus Biologicals, part of Bio-Techne
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NBP2-55604
NBP2-55604-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carbohydrate Sulfotransferase 2/CHST2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: HALGAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbohydrate Sulfotransferase 2/CHST2 Antibody

Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 2/CHST2 Antibody [NBP2-55604]

Immunocytochemistry/ Immunofluorescence: Carbohydrate Sulfotransferase 2/CHST2 Antibody [NBP2-55604]

Immunocytochemistry/Immunofluorescence: Carbohydrate Sulfotransferase 2/CHST2 Antibody [NBP2-55604] - Staining of human cell line PC-3 shows localization to the Golgi apparatus.

Applications for Carbohydrate Sulfotransferase 2/CHST2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbohydrate Sulfotransferase 2/CHST2

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. Human CHST2, also known as N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-2), was previously shown to act on non-reducing GlcNAc residues. The enzyme is known to be involved in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X and therefore plays a role in lymphocyte homing.

Alternate Names

C6ST, CHST2, GlcNAc6ST-1, GN6ST, GST-2

Gene Symbol

CHST2

Additional Carbohydrate Sulfotransferase 2/CHST2 Products

Product Documents for Carbohydrate Sulfotransferase 2/CHST2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbohydrate Sulfotransferase 2/CHST2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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