Carbonic Anhydrase I/CA1 Antibody (1F1)
Novus Biologicals, part of Bio-Techne | Catalog # H00000759-M10
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1F1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CA1 (AAH27890, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF
Specificity
CA1 (1F1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody (1F1)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Western-Blot-H00000759-M10-img0005.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]")
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in transfected 293T cell line by CA1 monoclonal antibody (M10), clone 1F1. Lane 1: CA1 transfected lysatE (28.9 KDa). Lane 2: Non-transfected lysate.![Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-I-CA1-Antibody-1F1-Western-Blot-H00000759-M10-img0004.jpg "Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]")
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10]
Western Blot: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Analysis of CA1 expression in human lung cancer.
Sandwich ELISA: Carbonic Anhydrase I/CA1 Antibody (1F1) [H00000759-M10] - Detection limit for recombinant GST tagged CA1 is approximately 0.1ng/ml as a capture antibody.
Applications for Carbonic Anhydrase I/CA1 Antibody (1F1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against tissue lysate for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase I/CA1
Additional Carbonic Anhydrase I/CA1 Products
Product Documents for Carbonic Anhydrase I/CA1 Antibody (1F1)
Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody (1F1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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