Carbonic Anhydrase I/CA1 Antibody (26E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26469
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 26E3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Carbonic Anhydrase I/CA1 [UniProt P00915]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody (26E3)
Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] -
Immunohistochemistry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Image of Carbonic Anhydrase I/CA1 Antibody (26E3) diluted at 1:50 and staining in paraffin-embedded human spleen tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.36% DAB.Western Blot: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] -
Western Blot: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Positive Western Blot detected in: K562 whole cell lysate, U937 whole cell lysate.All lanes: Carbonic Anhydrase I/CA1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] -
Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (26E3) [NBP3-26469] - Overlay Peak curve showing MCF-7 cells stained with Carbonic Anhydrase I/CA1 Antibody (26E3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for Carbonic Anhydrase I/CA1 Antibody (26E3)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase I/CA1
Alternate Names
CA1
Gene Symbol
CA1
Additional Carbonic Anhydrase I/CA1 Products
Product Documents for Carbonic Anhydrase I/CA1 Antibody (26E3)
Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody (26E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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