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Carbonic Anhydrase I/CA1 Antibody (S03-8F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19591

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-19591-100ul
NBP3-19591-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-8F1

Concentration

0.3 mg/ml

Product Summary for Carbonic Anhydrase I/CA1 Antibody (S03-8F1)

Immunogen

A synthetic peptide of human Carbonic Anhydrase I/CA1 (Uniprot # P00915)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody (S03-8F1)

Western Blot: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591]

Western Blot: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591]

Western Blot: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591] - Western blot detection of Carbonic Anhydrase I/CA1 in Rat Brain lysates using NBP3-19591 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591]

Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591]

Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody (S03-8F1) [NBP3-19591] - Immunohistochemistry of Carbonic Anhydrase I/CA1 in paraffin-embedded Human colon cancer tissue using NBP3-19591 at dilution 1/50

Applications for Carbonic Anhydrase I/CA1 Antibody (S03-8F1)

Application
Recommended Usage

Immunohistochemistry

1/50

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase I/CA1

Carbonic anhydrase performs an important role in respiration by facilitating transfer of CO2. Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Alternate Names

CA1

Gene Symbol

CA1

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Carbonic Anhydrase I/CA1 Antibody (S03-8F1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody (S03-8F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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