Carbonic Anhydrase I/CA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36740

Novus Biologicals, part of Bio-Techne
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NBP1-36740
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Carbonic Anhydrase I/CA1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QAIKTKGKRAP corresponding to internal region according to NP_001729.1.

Epitope

internal region

Specificity

Reported variants represent identical protein: NP_001729.1, NP_001122303.1, NP_001122302.1, NP_001122301.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody

Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740]

Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740]

Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740] - (0.03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740]

Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740]

Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-36740] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Click

Applications for Carbonic Anhydrase I/CA1 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
Western blot: Approx 29kDa band observed in Human Liver lysates (calculated MW of 28.9kDa according to NP_001729.1.Primary incubation was 1 hour.
IHC: Paraffin embedded Human Spleen.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase I/CA1

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature.

Alternate Names

CA1

Entrez Gene IDs

759 (Human); 12346 (Mouse); 310218 (Rat)

Gene Symbol

CA1

UniProt

NP_001729.1 (Human)

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Carbonic Anhydrase I/CA1 Antibody

Product Datasheets

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Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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