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Carbonic Anhydrase II/CA2 Antibody (001)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89522

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase II/CA2 (Accession#: NP_000058.1; Met1-Lys260).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody (001)

Western Blot: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522]

Western Blot: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522]

Western Blot: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDa Observed band size:30 kDa
Immunoprecipitation: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522]

Immunoprecipitation: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522]

Immunoprecipitation: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-CA2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CA2 rabbit monoclonal antibody,at 1:2000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size: 29 kDa

Applications for Carbonic Anhydrase II/CA2 Antibody (001)

Application
Recommended Usage

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:10000-1:50000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase II/CA2

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

Alternate Names

CA2

Gene Symbol

CA2

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Carbonic Anhydrase II/CA2 Antibody (001)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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