Carbonic Anhydrase II/CA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-919

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB600-919-100ug
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Conjugate
Catalog #
Biotin
NB200-607
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all Carbonic Anhydrase II/CA2 Antibodies »

Product Specifications

Immunogen

Carbonic Anhydrase II/CA2 Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II/CA2. (Uniprot: P00918)

Reactivity Notes

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Localization

Cytoplasmic

Specificity

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.
Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919]

Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.
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Applications for Carbonic Anhydrase II/CA2 Antibody

Application
Recommended Usage

ELISA

1:165000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA, western blot, and immunohistochemistry. Expect ~29.2kDa. Researchers should determine optimal titers for applications that are not stated below.

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID

Reviewed Applications

Read 3 reviews rated 5 using NB600-919 in the following applications:

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase II/CA2

Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Alternate Names

CA2

Gene Symbol

CA2

UniProt

P00918

Additional Carbonic Anhydrase II/CA2 Products

Product Documents for Carbonic Anhydrase II/CA2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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