Carbonic Anhydrase II/CA2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52736
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NBP2-52736-img0004.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLG
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25400751).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Carbonic Anhydrase II/CA2 Antibody
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-Paraffin-NBP2-52736-img0003.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]")
Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]
Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Staining of human duodenum shows nuclear positivity in glandular cells.![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NBP2-52736-img0001.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]")
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736]
Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla)
Applications for Carbonic Anhydrase II/CA2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase II/CA2
Alternate Names
CA2
Gene Symbol
CA2
Additional Carbonic Anhydrase II/CA2 Products
Product Documents for Carbonic Anhydrase II/CA2 Antibody
Product Specific Notices for Carbonic Anhydrase II/CA2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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