Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51691
![Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Flow-Cytometry-NBP1-51691-img0008.jpg "Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]")
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3)BSA Free [NBP1-51691]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Western-Blot-NBP1-51691-img0006.jpg "Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3)BSA Free [NBP1-51691]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2D3
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This Carbonic Anhydrase IX/CA9 Antibody (2D3) was made to a purified recombinant fragment of human Carbonic Anhydrase IX expressed in E. coli [UniProt# Q16790].
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 33153038).
Marker
Hypoxia Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free
Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3)BSA Free [NBP1-51691]
Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Carbonic Anhydrase IX mouse antibody against Hela (1) and A549 (2) cell lysates. Bands were detected at a molecular weight of approximately 50 kDa in both cell lines.![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Immunohistochemistry-Paraffin-NBP1-51691-img0005.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]")
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Paraffin-embedded lung tissues (left) and colonic tissues (right) using Carbonic Anhydrase IX mouse antibody with DAB staining.![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-51691-img0012.jpg "Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]")
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]
Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Carbonic Anhydrase IX/CA9 Antibody [2D3] (NBP1-51691) at 2ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Immunocytochemistry-Immunofluorescence-NBP1-51691-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]")
![Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-2D3-Flow-Cytometry-NBP1-51691-img0007.jpg "Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free [NBP1-51691]")
Applications for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow (Intracellular)
1 ug/mL
Flow Cytometry
1:200 - 1:400
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using NBP1-51691 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase IX/CA9
Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).
References
1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200
2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9
3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977
4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0
5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394
6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free
Product Specific Notices for Carbonic Anhydrase IX/CA9 Antibody (2D3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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