Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34408
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free [NBP2-34408]](https://resources.bio-techne.com/images/products/Carbonic-Anhydrase-IX-CA9-Antibody-SPM314-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34408-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free [NBP2-34408]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Equine
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM314
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free
Immunogen
Microsomal fraction of human renal cortical tissue homogenate
Localization
Cell surface and cytoplasmic
Specificity
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This monoclonal antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Marker
Renal Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34360).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free [NBP2-34408]
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free [NBP2-34408] - FFPE human renal cell carcinoma stained with RCC MAb (SPM314). Note cytoplasmic & cell surface staining of tumor cells.Applications for Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Carbonic Anhydrase IX/CA9
Carbonic anhydrase IX (theoretical molecular weight 50kDa) belongs to the monomeric alpha class and is a single pass-transmembrane protein with two extracellular domains which serve catalytic and cell adhesion functions (2, 3). By cooperating with sodium bicarbonate cotransporters (NBC), lactate and proton exporting monocarboxylic acid transporters (MCT), and a sodium/hydrogen exchanger (NHE), carbonic anhydrase IX is involved in pH regulation across the cell membrane. This functional property protects cancer cells from intracellular acidification and partly explains the role of carbonic anhydrase IX in cancer cell survival and proliferation. In contrast, the pH regulating activity of carbonic anhydrase IX induces extracellular acidification, which has been implicated in epithelial to mesenchymal transition (EMT) and promoting cancer invasion. Carbonic anhydrase IX is frequently overexpressed in cancer cells (e.g., colorectal-, breast-, lung-carcinoma and brain tumors), an effect promoted by hypoxia within the tumor microenvironment (4). An exception are tumors carrying pVHL inactivating mutations, such as clear cell renal cell carcinoma (ccRCC), where HIF-alpha is stabilized due to dysfunctional proteasomal targeting and can induce HRE (Hypoxia Response Element) containing genes even under physiological normoxia (5). Carbonic anhydrase IX may be detected by immunostaining in tumors, which is found in association with necrotic tissue and metastatic cells. Because the expression of carbonic anhydrase IX correlates with both tumor grade and stage, analysis of its expression in tumors serves as a prognostic factor (4, 6).
References
1. Tripp, B. C., Smith, K., & Ferry, J. G. (2001). Carbonic Anhydrase: New Insights for an Ancient Enzyme. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.R100045200
2. Nishimori, I., & Onishi, S. (2001). Carbonic anhydrase isozymes in the human pancreas. Digestive and Liver Disease. https://doi.org/10.1016/s1590-8658(01)80138-9
3. Zavadova, Z., & Zavada, J. (2005). Carbonic anhydrase IX (CA IX) mediates tumor cell interactions with microenvironment. Oncology Reports. https://doi.org/10.3892/or.13.5.977
4. Pastorekova, S., & Gillies, R. J. (2019). The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond. Cancer and Metastasis Reviews. https://doi.org/10.1007/s10555-019-09799-0
5. Haase, V. (2009). The VHL Tumor Suppressor: Master Regulator of HIF. Current Pharmaceutical Design. https://doi.org/10.2174/138161209789649394
6. Young, J. R., Coy, H., Kim, H. J., Douek, M., Sisk, A., Pantuck, A. J., & Raman, S. S. (2018). Association of the gross appearance of intratumoral vascularity at MDCT with the carbonic anhydrase IX score in clear cell renal cell carcinoma. American Journal of Roentgenology. https://doi.org/10.2214/AJR.18.19725
Alternate Names
CA9, G250, MN, RCC
Gene Symbol
CA9
Additional Carbonic Anhydrase IX/CA9 Products
Product Documents for Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free
Product Specific Notices for Carbonic Anhydrase IX/CA9 Antibody (SPM314) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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