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Carbonic Anhydrase VB/CA5B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86090

Novus Biologicals, part of Bio-Techne
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NBP1-86090
NBP1-86090-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GLAVIGVFLKLGKHHKELQKLVDTLPSIKHKDALVEFGSFDPSCLMPTCPDYWTYSGSLTTPPLSESVTWIIKKQPVEVDHDQLEQFRTLLFTSEGEKEKRMVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase VB/CA5B Antibody

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090] - Staining of human cell line HEK 293 shows localization to mitochondria. Antibody staining is shown in green.
Carbonic Anhydrase VB/CA5B Antibody

Western Blot: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090] -

Western Blot: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090] - Analysis in human cell line PC-3.
Carbonic Anhydrase VB/CA5B Antibody

Immunohistochemistry-Paraffin: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090] -

Immunohistochemistry-Paraffin: Carbonic Anhydrase VB/CA5B Antibody [NBP1-86090] -Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for Carbonic Anhydrase VB/CA5B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase VB/CA5B

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes responsible for catalyzing the reversible hydration of carbon dioxide.CAs show extensive diversity in their distribution and subcellular localization.They are involved in a variety of biological processes, including calcification, bone resorption, respiration, acid-base balance and the formation of aqueous humor, saliva, gastric juice and cerebrospinal fluid. CA VB, also known as carbonate dehydratase VB, is one of two isoforms of CA V. It localizes to the mitochondria and is involved in metabolic processes. CA VB is predominantly expressed in heart, pancreas, lung, placenta, kidney and skeletal muscle. It exhibits highest homology with family member CA VA (the second isoform of CA V); however, unlike CA VA, it is not expressed in the liver, suggesting that it plays a significantly different physiological role.

Alternate Names

CA5B, CAVB

Gene Symbol

CA5B

Additional Carbonic Anhydrase VB/CA5B Products

Product Documents for Carbonic Anhydrase VB/CA5B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Carbonic Anhydrase VB/CA5B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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