Carbonic Anhydrase VIII/CA8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98350

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Carbonic Anhydrase VIII/CA8 Antibodies »

Product Summary for Carbonic Anhydrase VIII/CA8 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase VIII/CA8 (Accession#: P28651; Met1-Gln291)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Carbonic Anhydrase VIII/CA8 Antibody

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350]

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350]

Western Blot: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Anti-Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).
Immunohistochemistry-Paraffin: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350]

Immunohistochemistry-Paraffin: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350]

Immunohistochemistry-Paraffin: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Staining of mouse Carbonic Anhydrase VIII/CA8 in mouse brain with rabbit polyclonal antibody at 1:25000 dilution.
Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350]

Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350]

Immunoprecipitation: Carbonic Anhydrase VIII/CA8 Antibody [NBP2-98350] - Mouse Carbonic Anhydrase VIII/CA8 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 0.5 ul anti-Mouse Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Carbonic Anhydrase VIII/CA8 rabbit polyclonal antibody, at 1:1000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 33 kDa. Observed band size: 37 kDa

Applications for Carbonic Anhydrase VIII/CA8 Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunohistochemistry

1:5000-1:25000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase VIII/CA8

Carbonic anhydrase-related protein (CARP) is a novel IP3R1-binding protein. Analysis of this protein revealed it is expressed exclusively in the Purkinje cells of the cerebellum.

Alternate Names

CA8, CALS, CARP

Gene Symbol

CA8

Additional Carbonic Anhydrase VIII/CA8 Products

Product Documents for Carbonic Anhydrase VIII/CA8 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase VIII/CA8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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