Carboxylesterase 1/CES1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00167
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for Carboxylesterase 1/CES1 Antibody
Immunogen
Peptide with sequence C-NTQAAQKLKDKE corresponding to internal region (near C-Terminus) according to NP_001020366.1, NP_001020365.1, NP_001257.4.
Specificity
This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Carboxylesterase 1/CES1 Antibody
![Western Blot: Carboxylesterase 1/CES1 Antibody [NBP1-00167]](https://resources.bio-techne.com/images/products/CES1-Antibody-Western-Blot-NBP1-00167-img0005.jpg "Western Blot: Carboxylesterase 1/CES1 Antibody [NBP1-00167]")
Western Blot: Carboxylesterase 1/CES1 Antibody [NBP1-00167]
Western Blot: CES1 Antibody [NBP1-00167] - Analysis of CES1 in Human Liver lysate (35ug protein in RIPA buffer) using NBP1-00167 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-00167]](https://resources.bio-techne.com/images/products/CES1-Antibody-Immunohistochemistry-Paraffin-NBP1-00167-img0004.jpg "Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-00167]")
Immunohistochemistry-Paraffin: Carboxylesterase 1/CES1 Antibody [NBP1-00167]
Immunohistochemistry-Paraffin: CES1 Antibody [NBP1-00167] - (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Carboxylesterase 1/CES1 Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 3 ug/ml
Immunohistochemistry-Paraffin
2 - 3 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 55-60 kDa band observed in human liver and Ileum lysates (calculated MW of 62.6 kDa band according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human Lung shows staining of lymphocytes in the vasculature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Carboxylesterase 1/CES1
Alternate Names
ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH
Entrez Gene IDs
1066 (Human)
Gene Symbol
CES1
UniProt
Additional Carboxylesterase 1/CES1 Products
Product Documents for Carboxylesterase 1/CES1 Antibody
Product Specific Notices for Carboxylesterase 1/CES1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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