Carboxylesterase 2/CES2 Antibody (4F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00008824-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Carboxylesterase 2/CES2 Antibodies »

Product Specifications

Immunogen

CES2 (NP_003860, 514 a.a. ~ 621 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHT

Specificity

CES2 (4F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Carboxylesterase 2/CES2 Antibody (4F12)

Western Blot: Carboxylesterase 2/CES2 Antibody (4F12) [H00008824-M02]

Western Blot: CES2 Antibody (4F12) [H00008824-M02] - CES2 monoclonal antibody (M02), clone 4F12 Analysis of CES2 expression in HepG2.

Immunohistochemistry-Paraffin: Carboxylesterase 2/CES2 Antibody (4F12) [H00008824-M02]

Immunohistochemistry-Paraffin: CES2 Antibody (4F12) [H00008824-M02] - Analysis of monoclonal antibody to CES2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Sandwich ELISA: CES2 Antibody (4F12) [H00008824-M02] - Detection limit for recombinant GST tagged CES2 is approximately 0.3ng/ml as a capture antibody.

Applications for Carboxylesterase 2/CES2 Antibody (4F12)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Carboxylesterase 2/CES2

Carboxylesterase 2 is a member of a large multigene family. The enzymes encoded by these genes are responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and heroin. They also hydrolize long-chain fatty acid esters and thioesters. The specific function of this enzyme has not yet been determined; however, it is speculated that carboxylesterases may play a role in lipid metabolism and/or the blood-brain barrier system. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

CE-2, CES2, PCE-2

Entrez Gene IDs

8824 (Human)

Gene Symbol

CES2

OMIM

605278 (Human)

UniProt

NP_003860 (Human)

Additional Carboxylesterase 2/CES2 Products

Product Documents for Carboxylesterase 2/CES2 Antibody (4F12)

Product Datasheets

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COA

SDS

Product Specific Notices for Carboxylesterase 2/CES2 Antibody (4F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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