Carboxypeptidase M Antibody (OTI2D11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46352

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D11

Concentration

1 mg/ml

View all Carboxypeptidase M Antibodies »

Product Summary for Carboxypeptidase M Antibody (OTI2D11)

Immunogen

Human recombinant protein fragment corresponding to amino acids 124-423 of human CPM(NP_001865) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carboxypeptidase M Antibody (OTI2D11)

Western Blot: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352]

Western Blot: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352]

Western Blot: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPM ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiCPM.(1:2000).
Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352]

Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352]

Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352] - Staining of paraffin-embedded Human liver tissue within the normal limits using antiCPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)
Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352]

Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352]

Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody (OTI2D11) [NBP2-46352] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CPM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min) (1:150)
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Applications for Carboxypeptidase M Antibody (OTI2D11)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Carboxypeptidase M

The protein encoded by the Carboxypeptidase M gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Alternate Names

CPM

Gene Symbol

CPM

Additional Carboxypeptidase M Products

Product Documents for Carboxypeptidase M Antibody (OTI2D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carboxypeptidase M Antibody (OTI2D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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