Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all CARD10/CARMA3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10. The immunogen is located within the last 50 amino acids of CARD10. Amino Acid Squence: KECGSSRGCPSSSED

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD10 Antibody - BSA Free

Western Blot: CARD10 AntibodyBSA Free [NBP1-76676]

Western Blot: CARD10 AntibodyBSA Free [NBP1-76676]

Western Blot: CARD10 Antibody [NBP1-76676] - Analysis of CARD10 in A-20 cell lysate with CARD10 antibody at 5 ug/mL.
CARD10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD10 Antibody - BSA Free [NBP1-76676] -

Immunocytochemistry/ Immunofluorescence: CARD10 Antibody - BSA Free [NBP1-76676] - Immunofluorescence of CARD10 in EL4 cells with CARD10 antibody at 20 u/mL.
CARD10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD10 Antibody - BSA Free [NBP1-76676] -

Immunocytochemistry/ Immunofluorescence: CARD10 Antibody - BSA Free [NBP1-76676] - Immunocytochemistry of CARD10 in EL4 cells with CARD10 antibody at 10 ug/mL.

Applications for CARD10 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

5 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD10/CARMA3

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-kappaB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IkappaK complex, demonstrating its importance in the regulation of NF-kappaB transcription factor activation.

Long Name

Caspase Recruitment Domain-containing Protein 10

Alternate Names

BIMP1, CARMA3

Gene Symbol

CARD10

UniProt

AAK26165 (Human)

Additional CARD10/CARMA3 Products

Product Documents for CARD10 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CARD10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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