CARD11/CARMA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1200

Novus Biologicals, part of Bio-Techne
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NB100-1200
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CARD11/CARMA1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QRKTIWVDEDQL corresponding to C-Terminus according to NP_115791.3.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24036998).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CARD11/CARMA1 Antibody

Western Blot: CARD11/CARMA1 Antibody [NB100-1200]

Western Blot: CARD11/CARMA1 Antibody [NB100-1200]

Western Blot: CARD11/CARMA1 Antibody [NB100-1200] - Staining of Human Jurkat cells (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [NB100-1200]

Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [NB100-1200]

Immunohistochemistry-Paraffin: CARD11/CARMA1 Antibody [NB100-1200] - Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for CARD11/CARMA1 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 5 ug/ml

Immunohistochemistry-Paraffin

2-5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. IHC: Paraffin embedded human spleen shows nuclear staining.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CARD11/CARMA1

This protein belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

Long Name

Caspase Recruitment Domain-containing Protein 11

Alternate Names

BIMP3, CARMA1

Entrez Gene IDs

84433 (Human); 108723 (Mouse)

Gene Symbol

CARD11

UniProt

NP_115791. (Human)

Additional CARD11/CARMA1 Products

Product Documents for CARD11/CARMA1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CARD11/CARMA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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