CARD12 [p Ser533] Antibody (4B7B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27120

Novus Biologicals, part of Bio-Techne
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NBP3-27120-0.025ml
NBP3-27120-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B7B7

Concentration

1 mg/ml

View all CARD12 Antibodies »

Product Specifications

Immunogen

Synthesized peptide of mouse CARD12 [p Ser533] (AA: 525-538) expressed in E. Coli.

Modification

p Ser533

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD12 [p Ser533] Antibody (4B7B7)

Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (4B7B7) [NBP3-27120] -

Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (4B7B7) [NBP3-27120] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western Blot: CARD12 [p Ser533] Antibody (4B7B7) [NBP3-27120] -

Western Blot: CARD12 [p Ser533] Antibody (4B7B7) [NBP3-27120] - Analysis using CARD12 (p Ser533) rat mAb against NIH/3T3 (1) cell lysate.

ELISA: CARD12 [p Ser533] Antibody (4B7B7) [NBP3-27120] -

ELISA: CARD12 [p Ser533] Antibody (4B7B7) [NBP3-27120] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
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Applications for CARD12 [p Ser533] Antibody (4B7B7)

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD12

Ipaf (also known as Clan/CARD12) is a CARD domain containing protein. CARD (caspase-associated recruitment domain) proteins are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). In general CARD proteins are implicated in host defense against infection, environmental stress or cellular damage. CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including Ipaf/Clan/CARD12. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. There are at least three major signaling pathways in which CARD proteins act: (1) Regulation of caspase activation in the context of apoptosis (2) Regulation of caspase activation in the context of inflammation (3) Regaultion of NF-kB activation in the context of innate or adaptive immune responses. As there is significant crosstalk between pathways that lead to caspase-mediated apoptosis or inflammation and pathways that result in NF-kB activation, it is logical that similar protein modules such as CARD domains are found repeatedly in proteins from all three pathways. Ipaf plays a role in regulating caspase-1 activity, which in turn mediates the maturation of inflammatory cytokines IL-1b and IL-18 (reviewed in Lu et al, 2005). In transfected cells, Ipaf has been shown to directly interact with procaspase-1 and induce proteolytic activation of procaspase-1 in transfected cells. On the flip side, macrophages from IPAF deficient mice failed to activate caspase-1 in response to Salmonella typhimurium infection underscoring the importance of IPAF in vivo. IPAF also interact with the pro-apoptotic adaptor protein ASC and co-expression of IPAF with ASC has been shown to induce NF-kB activation and apoptosis.

Alternate Names

CARD12leucine rich repeat and CARD domaincontaining 4, caspase recruitment domain family, member 12, Caspase recruitment domain-containing protein 12, Clan protein, CLAN1ipaf, CLANCARD, LRR, and NACHT-containing protein, CLR2.1, Ice protease-activating factor, Ipaf, NLR family, CARD domain containing 4

Gene Symbol

NLRC4

Additional CARD12 Products

Product Documents for CARD12 [p Ser533] Antibody (4B7B7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CARD12 [p Ser533] Antibody (4B7B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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