CARD12 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78979

Novus Biologicals, part of Bio-Techne
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NBP1-78979
NBP1-78979SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.13 mg/ml

View all CARD12 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human CARD12 protein (between residues 600-700) [UniProt Q9NPP4]

Localization

Cytoplasm. Note: Cytoplasmic filaments.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CARD12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD12 Antibody - BSA Free [NBP1-78979]

Immunocytochemistry/ Immunofluorescence: CARD12 Antibody - BSA Free [NBP1-78979]

Immunocytochemistry/Immunofluorescence: CARD12 Antibody [NBP1-78979] - The CARD12 antibody (NBP1-78979) was tested in HepG2 cells at a 1:250 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Immunohistochemistry: CARD12 Antibody - BSA Free [NBP1-78979]

Immunohistochemistry: CARD12 Antibody - BSA Free [NBP1-78979]

Immunohistochemistry: CARD12 Antibody [NBP1-78979] - IHC analysis of CARD12 in mouse lung using DAB with hematoxylin counterstain.

Applications for CARD12 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 29104144)

Immunoblotting

reported in scientific literature (PMID 29104144)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.13 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARD12

CARD12, also known as CLAN, IPAF and CLAN1 is a member of the CED4 family of proteins. The CED4/Apaf-1 proteins are critical regulators of apoptosis and NF-kappaB signaling pathways, and CARD12 is no exception. CARD12 has been implicated in coordination of downstream activation of both apoptotic and inflammatory signaling pathways (PMID: 11374873). Additionally, CARD12 has shown to coprecipitate with caspase 1, an enzyme that participates in both the signaling of apoptosis and cytokine processing, further demonstrating its integral role in programmed cell death (PMID: 11374873). As part of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) proteins, CARD12 is known to be an important regulator of caspase-1 activation via an ATP/dATP nucleotide-binding pocket (PMID: 15882992). CARD12 has four known isoforms. Isoform 1 is highly expressed in lung, followed by leukocytes (especially monocytes), lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Although it is expressed ubiquitously, Isoform 2 is more prominent at the highest levels in lung and spleen. Isoform 4 can only been found in the brain.

Alternate Names

CARD12leucine rich repeat and CARD domaincontaining 4, caspase recruitment domain family, member 12, Caspase recruitment domain-containing protein 12, Clan protein, CLAN1ipaf, CLANCARD, LRR, and NACHT-containing protein, CLR2.1, Ice protease-activating factor, Ipaf, NLR family, CARD domain containing 4

Entrez Gene IDs

58484 (Human)

Gene Symbol

NLRC4

UniProt

Q9NPP4 (Human)

Additional CARD12 Products

Product Documents for CARD12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CARD12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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