CARD6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15704
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Electron Microscopy, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Electron Microscopy, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human CARD6. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CARD6 Antibody
Immunohistochemistry: CARD6 Antibody [NBP2-15704]
Immunohistochemistry: CARD6 Antibody [NBP2-15704] - Induced expression of NOD2, CARD6, PYCARD and IFI16 in psoriasis lesions. Immunohistochemistry shows stronger NOD2, CARD6, PYCARD and IFI16 staining in PL (upper) than in controls (lower). PN (middle) and C are almost negative for NOD2 and CARD6. IFI16 is predominantly in nuclei of PL and PN. In controls IFI16 expression is weak and cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL and PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep22745) licensed under a CC-BY license.Western Blot: CARD6 Antibody [NBP2-15704]
Western Blot: CARD6 Antibody [NBP2-15704] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.Immunocytochemistry/ Immunofluorescence: CARD6 Antibody [NBP2-15704]
Immunocytochemistry/Immunofluorescence: CARD6 Antibody [NBP2-15704] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.Applications for CARD6 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Application Notes
Electron microscopy usage reported in scientific literature (PMID: 26976200).
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CARD6
Long Name
Caspase Recruitment Domain-containing Protein 6
Alternate Names
CINCIN1
Gene Symbol
CARD6
UniProt
Additional CARD6 Products
Product Documents for CARD6 Antibody
Product Specific Notices for CARD6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.