Carm1 Antibody (3H2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37645

Novus Biologicals, part of Bio-Techne
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NBP2-37645
NBP2-37645-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Monkey, Primate

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3H2

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Carm1 Antibodies »

Product Summary for Carm1 Antibody (3H2) - BSA Free

Immunogen

Purified recombinant fragment of human CARM1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Carm1 Antibody (3H2) - BSA Free

Western Blot: Carm1 Antibody (3H2)BSA Free [NBP2-37645]

Western Blot: Carm1 Antibody (3H2)BSA Free [NBP2-37645]

Western Blot: Carm1 Antibody (3H2) [NBP2-37645] - Western blot analysis using CARM1 mouse mAb against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: Carm1 Antibody (3H2) - BSA Free [NBP2-37645]

Immunocytochemistry/ Immunofluorescence: Carm1 Antibody (3H2) - BSA Free [NBP2-37645]

Immunocytochemistry/Immunofluorescence: Carm1 Antibody (3H2) [NBP2-37645] - Immunofluorescence analysis of Hela cells using CRAM1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: Carm1 Antibody (3H2) - BSA Free [NBP2-37645]

Immunohistochemistry: Carm1 Antibody (3H2) - BSA Free [NBP2-37645]

Immunohistochemistry: Carm1 Antibody (3H2) [NBP2-37645] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using CARM1 mouse mAb with DAB staining.
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Applications for Carm1 Antibody (3H2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carm1

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al., 2002 (PubMed 11724789)).(supplied by OMIM)

Long Name

Coactivator-associated Arginine Methyltransferase 1

Alternate Names

PRMT4

Gene Symbol

CARM1

Additional Carm1 Products

Product Documents for Carm1 Antibody (3H2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Carm1 Antibody (3H2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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