Carm1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB200-341
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for Carm1 Antibody
Immunogen
A synthetic peptide that maps to a region between residues 450 and 500 of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Carm1 Antibody
Western Blot: Carm1 Antibody [NB200-341]
Western Blot: Carm1 Antibody [NB200-341] - Detection of Human and Mouse CARM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody NB200-341 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341]
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341] - Human stomach carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).Western Blot: Carm1 Antibody [NB200-341]
Western Blot: Carm1 Antibody [NB200-341] - Whole HeLa cell lysate (25 mcg for WB in A; 5 mg for IP in B).antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP. Detection: Chemiluminescence A. Coomassie stain in B. Band was verified by mass spectrometry.Applications for Carm1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
1-4 ug/mg lysate
Western Blot
1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Carm1
Long Name
Coactivator-associated Arginine Methyltransferase 1
Alternate Names
PRMT4
Entrez Gene IDs
10498 (Human)
Gene Symbol
CARM1
UniProt
Additional Carm1 Products
Product Documents for Carm1 Antibody
Product Specific Notices for Carm1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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