Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2E2

Format

BSA Free

Concentration

1.0 mg/ml

View all Casein Kinase 1 alpha Antibodies »

Product Specifications

Immunogen

Recombinant human Casein Kinase 1 alpha (1-337aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Casein Kinase 1 alpha Antibody (2E2) - BSA Free

Western Blot: Casein Kinase 1 alpha Antibody (2E2)BSA Free [NBP1-30165]

Western Blot: Casein Kinase 1 alpha Antibody (2E2)BSA Free [NBP1-30165]

Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) - BSA Free [NBP1-30165]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) - BSA Free [NBP1-30165]

Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Analysis of Casein Kinase 1 alpha in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Casein Kinase 1 alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: Casein Kinase 1 alpha Antibody (2E2)BSA Free [NBP1-30165]

Western Blot: Casein Kinase 1 alpha Antibody (2E2)BSA Free [NBP1-30165]

Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Lane 1.: Jurkat cell lysate. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for Casein Kinase 1 alpha Antibody (2E2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Casein Kinase 1 alpha

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, beta, gamma, e and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The a catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Alternate Names

CSNK1A1, HLCDGP1

Entrez Gene IDs

1452 (Human)

Gene Symbol

CSNK1A1

UniProt

NP_001883 (Human)

Additional Casein Kinase 1 alpha Products

Product Documents for Casein Kinase 1 alpha Antibody (2E2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Casein Kinase 1 alpha Antibody (2E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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