Casein Kinase 2 alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-377
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0005.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]")
![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0012.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]")
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Chicken (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Casein Kinase 2 alpha Antibody
![Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Western-Blot-NB100-377-img0011.jpg "Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]")
Western Blot: Casein Kinase 2 alpha Antibody [NB100-377]
Western Blot: Casein Kinase 2 alpha Antibody [NB100-377] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha antibody NB100-377 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]](https://resources.bio-techne.com/images/products/Casein-Kinase-2-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-377-img0013.jpg "Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]")
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377]
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-377] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:1,000 (1ug/ml). Detection: DABApplications for Casein Kinase 2 alpha Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 -1:2000
Immunohistochemistry-Paraffin
1:500 -1:2000
Western Blot
1:2000 - 1:10000
Application Notes
By WB, this antibody recognizes a band at ~42 kDa, representing CKII alpha. Not recommended for immunoprecipitation. *NB 100-378 is recommended for IP. The investigator should determine the optimal working dilution for a specific application. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-buffered Saline, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Casein Kinase 2 alpha
Additional Casein Kinase 2 alpha Products
Product Documents for Casein Kinase 2 alpha Antibody
Product Specific Notices for Casein Kinase 2 alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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