Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Rabbit (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Casein Kinase 2 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and the C-terminus (residue 391) of human Casein Kinase 2, alpha 1 using the numbering given in entry NP_001886.1 (GeneID 1457).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Casein Kinase 2 alpha Antibody

Western Blot: Casein Kinase 2 alpha Antibody [NB100-378]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-378]

Western Blot: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of Human and Mouse CKII alpha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378]

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378]

Immunohistochemistry-Paraffin: Casein Kinase 2 alpha Antibody [NB100-378] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CKII Alpha used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378]

Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378]

Immunoprecipitation: Casein Kinase 2 alpha Antibody [NB100-378] - Detection of human CKII alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKII alpha antibody NB100-378 (lot NB100-378-2) used for IP at 6 ug/mg lysate. CKII alpha was also immunoprecipitated by a previous lot (lot NB100-378-1) of this antibody. For blotting immunoprecipitated CKII alpha, NB100-378 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for Casein Kinase 2 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000- 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Casein Kinase 2 alpha

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Alternate Names

CK2A1, CSNK2A1

Entrez Gene IDs

1457 (Human)

Gene Symbol

CSNK2A1

UniProt

NP_001886.1 (Human)

Additional Casein Kinase 2 alpha Products

Product Documents for Casein Kinase 2 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Casein Kinase 2 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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