Casein Kinase 2 beta Antibody (9T5J10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16264

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16264-100ul
NBP3-16264-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9T5J10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Casein Kinase 2 beta Antibodies »

Product Summary for Casein Kinase 2 beta Antibody (9T5J10)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 101-215 of human Casein Kinase 2 beta (CSNK2B) (P67870). YQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLYGFKIHPMAYQLQLQAASNFKSPVKTIR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Casein Kinase 2 beta Antibody (9T5J10)

Western Blot: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264]

Western Blot: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264]

Western Blot: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Western blot analysis of extracts of various cell lines, using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264]

Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264]

Immunocytochemistry/Immunofluorescence: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Immunofluorescence analysis of U-2 OS cells using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264]

Immunohistochemistry-Paraffin: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264]

Immunohistochemistry-Paraffin: Casein Kinase 2 beta Antibody (9T5J10) [NBP3-16264] - Mouse spinal cord using Casein Kinase 2 beta (CSNK2B) Rabbit mAb (NBP3-16264) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Casein Kinase 2 beta Antibody (9T5J10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Casein Kinase 2 beta

Casein Kinase 2 beta encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq]

Alternate Names

CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin

Gene Symbol

CSNK2B

Additional Casein Kinase 2 beta Products

Product Documents for Casein Kinase 2 beta Antibody (9T5J10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Casein Kinase 2 beta Antibody (9T5J10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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