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Caspase-11 Antibody (4E11) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80100CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Flow Cytometry, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG1 kappa Clone # 4E11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant mouse caspase-11 (p20 fragment).

Specificity

Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1 kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Caspase-11 Antibody (4E11) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Caspase-11

Caspase-11 (also known as CASP11, CASP4, and ich-3) is one of several different types of caspases. The general role of caspases is to assist in cell death as well as inflammation. More specifically, caspase-11 is involved with pyroptotic cell death. Its structure is heterotrimeric, consisting of two antiparallel heterodimers. Novus offers Caspase 11 antibodies, Peptides and Proteins, Lysates, and RNAi.

Alternate Names

CASP11, CASP-11, CASP4, CASP-4, caspase 4, apoptosis-related cysteine peptidase, Caspl, ich-3

Gene Symbol

Casp4

Additional Caspase-11 Products

Product Documents for Caspase-11 Antibody (4E11) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Caspase-11 Antibody (4E11) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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