Caspase-12 Antibody (14F7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1432
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 14F7
Format
BSA Free
Concentration
2.0 mg/ml
Product Specifications
Immunogen
Recombinant fragment: GPQKICTPSS PSESKRKVEDD EMEVNAGLAH ESHLM LTAPH GLQSSEVQDTL KLCPRDQFCK IKTERAKEIY PVMEKEGRTRL ALIICNKKFDY LFDRDNADTDI LNMQELLENLG YSVVLKENLTA QEMETELMQFA GRPEHQSSDST FLVFMSHGIL EGICGVKHRNK KPDVLHDDTIF KIFNNSNCRSL RNKPKILIMQA CRGRYNGTIWV STNKGIATAD, corresponding to amino acids 95-318 of Mouse Caspase 12.
Localization
Endoplasmic reticulum
Specificity
This antibody recognizes the full length and possibly the truncated p20 subunit of Caspase 12.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Caspase-12 Antibody (14F7) - BSA Free
![Western Blot: Caspase-12 Antibody (14F7)BSA Free [NB600-1432]](https://resources.bio-techne.com/images/products/Caspase-12-Antibody-14F7-BSA-Free-Western-Blot-NB600-1432-img0002.jpg "Western Blot: Caspase-12 Antibody (14F7)BSA Free [NB600-1432]")
Western Blot: Caspase-12 Antibody (14F7)BSA Free [NB600-1432]
Western Blot: Caspase-12 Antibody (14F7) - BSA Free [NB600-1432] - NRK Rat kidney cell extract was separated on SDS-PAGE and probed with Monoclonal (RAT) AB TO CASPASE-12 CL:14F7. The antibody was developed with HRP APA Rat IgG and an ECL substrate. Antibody concentration 1ug/mL.Applications for Caspase-12 Antibody (14F7) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1-2 ug/ml
Application Notes
Immunocytochemistry: 4% paraformaldehyde-fixed, paraffin-embedded). Predicted molecular weight: 50 kDa. Antibody may also work in Immunohistochemistry and immunoprecipitation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
2.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Caspase-12
Additional Caspase-12 Products
Product Documents for Caspase-12 Antibody (14F7) - BSA Free
Product Specific Notices for Caspase-12 Antibody (14F7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...